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 News info
We are happy to inform you that Erwin Kaal has won the 3th price Poster Award at HTC-11.
Finding biomarkers for fast detection of Mycobacterium tuberculosis in sputum samples using THM-GC-MS
Come and visit us @ HTC-11, Bruges , Belgium
January 26th – 27th, 2010
For more information go here.
New OPTIC Publication , January 2010.
Metabolic Profiling of Ultrasmall Sample Volumes
with GC/MS: From Microliter to Nanoliter Samples
Maud M. Koek, Floor Bakels, Willem Engel, Arn van den Maagdenberg, Michel D. Ferrari, Leon Coulier and Thomas Hankemeier. Anal. Chem., 2010, 82 (1), pp 156–16
<< Read Abstract >>
Profiling of metabolites is increasingly used to study
the functioning of biological systems. For some studies the volume of available samples is limited to only a few microliters
or even less, for fluids such as cerebrospinal fluid (CSF) of small animals like mice or the analysis of individual oocytes.
Here we present an analytical method using in-liner silylation coupled to gas chromatography/mass spectrometry (GC/MS),
that is suitable for metabolic profiling in ultrasmall sample volumes of 2 µL down to 10 nL. Method performance
was assessed in various biosamples. Derivatization efficiencies for sugars, organic acids, and
amino acids were satisfactory (105-120%), and repeatabilities were generally better than 15%, except for amino acids
that had repeatabilities up to about 35-40%. For endogenous sugars and organic acids in fetal bovine serum, the response
was linear for aliquots from 10 nL up to at least 1 µL. The developed GC/MS method was applied for the analysis of different s
ample matrixes, i.e., fetal bovine serum, mouse CSF, and aliquots of the intracellular content of Xenopus laevis oocytes.
To the best of our knowledge, we present here the first comprehensive GC/MS metabolite profiles from mouse CSF and from
the intracellular content of a single X. laevis oocyte.
2 new publications of the PHASER:
- Character impact odorants from Brassicaceae by aroma extract dilution analysis (AEDA): Brassica cretica and Brassica insularis
Katharina Breme, Stéphane Langle, Xavier Fernandez, Uwe J. Meierhenrich, Hugues Brevard, Daniel Joulain Flavour Frag. J. 2009, 24, 88-93
<< Read Abstract >>
Volatile extracts of two wild cabbages, Brassica cretica and Brassica insularis, were studied by GC-MS, GC/FID, and
GC-olfactometry/aroma extract dilution analysis (GC-O/AEDA). Major compounds were 3-butenyl cyanide (70% of the total GC/FID area)
and 3-butenyl isothiocyanate (29%) in B. cretica and 2-phenylethyl cyanide (53%) and 2-phenylethyl isothiocyanate (21%) in B. insularis.
AEDA revealed the impact of five heteroatomic trace compounds (diacetyl, methional, furfuryl thiol, 1-octen-3-one and skatole)
with moderate to high flavour dilution (FD) factors that were detected only due to GC-O analyses
- Identification of Odor Impact Compounds of Tagetes minuta L. Essential Oil: Comparison of Two GC-Olfactometry Methods
Katharina Breme, Pascal Tournayre, Xavier Fernandez, Uwe J. Meierhenrich, Hugues Brevard, Daniel Joulain and Jean Louis Berdagué J. Agric. Food Chem., 2009, 57 (18), pp 8572–8580
<< Read Abstract >>
Odor impact compounds of Tagetes minuta L. essential oil were studied by gas chromatography (GC)-olfactometry using aroma extract
dilution analysis (AEDA) and vocabulary-intensity-duration of elementary odors by sniffing (VIDEO-Sniff). AEDA was conducted by
direct injection and revealed the presence of 43 odorant zones. Highest flavor dilution (FD) values were obtained for ethyl 2-methylpropanoate,
ethyl 3-methylbutanoate, (E)-ocimenone, two tentatively identified thiols, and two yet unknown compounds. VIDEO-Sniff was realized by dynamic
headspace sampling (D-HS) combined with 8W-GC-olfactometry where eight sniffers simultaneously detect volatile compounds obtained from a single
chromatographic separation and revealed the presence of 42 odorant zones. Odorant trace compounds detected by GC-O that were present in
quantities inferior to the GC-qMS system’s detection limit and those subject to coelutions were identified by GC×GC-time-of-flight mass
spectrometry (TOFMS). A total amount of 37 odorant components could be identified by VIDEO-Sniff, and the strong influence of the fruity
notes of numerous esters stood out. Highest olfactory signals were obtained for ethyl 2-methylpropanoate, ethyl 2- and 3-methylbutanoate,
and oct-1-en-3-one. Both methods hence come to the conclusion that ethyl 2-methylpropanoate and ethyl 2- and 3-methylbutanoate are among the
main odorants in Tagetes minuta L. essential oil. Differences, advantages, and drawbacks of both GC-O methods are discussed.
To PHASER.
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