ATAS GL news: GC and HPLC New applications, publications, software updates, products etc… http://www.atasgl.com New applications, publications updates etc. en mirabyte Feed Writer 1.0 Fri, 03 Jul 2009 21:57:56 +0100 http://www.atasgl.com/feed/atasgl.gif ATAS GL news: GC and HPLC New applications, publications, software updates, products etc… http://www.atasgl.com 144 52 ATAS GL logo Analytical Science GC HPLC HTC-11 Poster Award We are happy to inform you that Erwin Kaal has won the 3th price Poster Award at HTC-11. http://www.atasgl.com/poster/HTC-11_Poster-TB_ATASGL.pdf Tue, 09 Feb 2010 12:44:00 +0100 Metabolic Profiling of Ultrasmall Sample Volumes Profiling of metabolites is increasingly used to study the functioning of biological systems. For some studies the volume of available samples is limited to only a few microliters or even less, for fluids such as cerebrospinal fluid (CSF) of small animals like mice or the analysis of individual oocytes. Here we present an analytical method using in-liner silylation coupled to gas chromatography/mass spectrometry (GC/MS), that is suitable for metabolic profiling in ultrasmall sample volumes of 2 μL down to 10 nL. Method performance was assessed in various biosamples. Derivatization efficiencies for sugars, organic acids, and amino acids were satisfactory (105−120%), and repeatabilities were generally better than 15%, except for amino acids that had repeatabilities up to about 35−40%. For endogenous sugars and organic acids in fetal bovine serum, the response was linear for aliquots from 10 nL up to at least 1 μL. The developed GC/MS method was applied for the analysis of different sample matrixes, i.e., fetal bovine serum, mouse CSF, and aliquots of the intracellular content of Xenopus laevis oocytes. To the best of our knowledge, we present here the first comprehensive GC/MS metabolite profiles from mouse CSF and from the intracellular content of a single X. laevis oocyte. http://www.atasgl.com/html/optic_pub.html#metabolic Thu, 14 Jan 2010 09:15:40 +0100 New catalogue ATAS GL is introducing a new catalogue with spare parts and consumables for the ATAS GL Instruments. Because of the many pictures in the catalogue it is easy to identify the part you need. http://www.atasgl.com/download/ATAS%20GL%20Supplies%20and%20accessories%202009_2010.pdf Tue, 10 Nov 2009 11:13:14 +0100 New menu on website We have changed our menu structure in order to make the website more convenient. www.atasgl.com Mon, 09 Nov 2009 16:57:11 +0100 Automated fatty acid profiling of raw algae’s by THM-GC-MS New application note: Automated fatty acid profiling of raw algae’s by THM-GC-MS http://www.atasgl.com/ap-note/111_profiling%20of%20raw%20algae’s.pdf Mon, 19 Oct 2009 15:57:26 +0100 New poster: 'Determination of non polar metabolites in human plasma’ Determination of non polar metabolites in human plasma using innovative sample preparation strategies coupled with gas chromatography and mass spectrometry http://www.atasgl.com/monotrap/poster_bremen_398.pdf Tue, 29 Sep 2009 10:51:46 +0100 New PowerPoint with MonoTrap information and applications New approach of simple pretreatment for GCMS analysis by using monolithic material sorptiveextraction http://www.atasgl.com/monotrap/GCMS_CF_CJK2009_MonoTrap_2009-09.pdf Wed, 16 Sep 2009 15:55:58 +0100 A fast method for the identification of Mycobacterium tuberculosis in sputum... A fast method for the identification of Mycobacterium tuberculosis in sputum and cultures based on thermally assisted hydrolysis and methylation followed by gas chromatography–mass spectrometry.

 Journal of Chromatography A, 1216 (2009) 6319–6325

 a b s t r a c t

 A fast gas chromatography–mass spectrometry (GC–MS) method with minimum sample preparation is described for early diagnosis of tuberculosis (TB). The automated procedure is based on the injection of sputum samples which are thenmethylated inside the GC injector using thermally assisted hydrolysis and methylation (THM). The THM–GC–MS procedure was optimized for the injection of sputum samples. For the identification of Mycobacterium tuberculosis the knownmarker tuberculostearic acid (TBSA) and other potential markers were evaluated. Hexacosanoic acid in combination with TBSA was found to be specific for the presence of M. tuberculosis. For validation of the method several sputum samples with different viscosities spiked with bacterial cultures were analyzed. Finally, 18 stored sputum samples collected in Vietnam from patients suspected to suffer from TB were re-analyzed in Amsterdam by microscopy after decontamination/concentration and using the new THM–GC–MS method. No false positives were found by THM–GC–MS and all patients who were diagnosed with TB were also found positive using our newly developed THM–GC–MS method. These results showthat the newfast and sensitive THM–GC–MS method holds great potential for the diagnosis of TB.

]]> Thu, 06 Aug 2009 18:17:57 +0100 International newsletter Our international newsletter number 2 for 2009 is out. http://www.atasgl.com/newsletter/newsletter_2009_2.html Tue, 04 Aug 2009 16:22:26 +0100 ISEO 2009 40th International Symposium on Essential Oils. ATAS GL and GL Sciences can be found on this symposium with a booth. Please visit us from September 6 till 9 2009. http://www.iseo2009.unito.it/ Wed, 22 Jul 2009 15:22:26 +0100 New application note: Pyrolysis of solid PE and PPSU Pyrolysis of four samples named A,B,C and D. Samples A and B are PE samples and C and D are PPSU samples. The request from the customer is to show the repeatability of pyrolysis performed with the OPTIC for all the samples, especially for sample A and C. http://www.atasgl.com/ap-note/110_Pyrolysis%20of%20solid%20PE%20and%20PPSU.pdf Wed, 22 Jul 2009 16:09:20 +0100 MonoTrap, a new trapping material MonoTrap is an easy consumable for trapping components and use it as sample enrichment device. http://www.atasgl.com/monotrap/MonoTrap_3.pdf http://www.atasgl.com/monotrap/MonoTrap_3.pdf Sat, 04 Jul 2009 00:02:55 +0100 Inertsil ODS 4 GL Sciences has improved the C18 column, their new name is ODS 4. http://www.inertsil.nl/ods4/ods-4.pdf http://www.inertsil.nl/ods4/ods-4.pdf Fri, 03 Jul 2009 23:00:50 +0100 Pyrolysis GCMS: New brochure for Pyrolysis GCMS with the OPTIC 3. Because of new publications. http://www.atasgl.com/download/Brochure%20OPTIC%203%20pyroliser.pdf http://www.atasgl.com/download/Brochure%20OPTIC%203%20pyroliser.pdf Fri, 03 Jul 2009 23:58:41 +0100